fluorene 9 bisphenol

In the evolving landscape of chemical analysis, the demand for highly sensitive and selective reagents is paramount. Among these, Fluorexon, also known by its CAS number 1461-15-0 , stands out as a critical fluorimetric indicator and chelating agent. Its unique properties enable precise detection and quantification across a spectrum of industrial and research applications. This comprehensive guide explores Fluorexon's scientific underpinnings, its intricate manufacturing process, diverse applications, and why Moneidechem's commitment to quality makes us a leading supplier in this specialized field. Industry Trends and the Growing Importance of Fluorexon The global market for analytical reagents is experiencing robust growth, driven by advancements in biotechnology, pharmaceutical research, environmental monitoring, and materials science. Within this segment, fluorescent indicators like Fluorexon are gaining prominence due to their superior sensitivity compared to traditional colorimetric methods. The ability of Fluorexon to form highly fluorescent complexes with various metal ions, particularly alkaline earth metals and some transition metals, makes it indispensable for: Environmental Monitoring: Increasing stringency in water quality regulations necessitates accurate detection of trace metal contaminants. Pharmaceutical Quality Control: Ensuring purity and precise composition of drug formulations. Biochemical Research: Studying enzyme kinetics, protein-ligand interactions, and cellular processes where metal ions play crucial roles. Industrial Process Control: Real-time monitoring of metal ion concentrations in manufacturing, such as in electroplating baths or boiler feed water. The trend towards miniaturization of analytical systems and the development of portable detection devices further amplifies the need for stable, high-performance fluorescent indicators, solidifying the position of Fluorexon as a key component in modern analytical chemistry. Figure 1: Conceptual representation of Fluorexon's versatile applications across various sectors. Technical Parameters and Specifications of Fluorexon (CAS: 1461-15-0) Fluorexon, also known as Calcein or o,o'-Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein, is a highly specialized chemical compound. Its efficacy in various applications is directly linked to its precise chemical and physical properties. Understanding these parameters is crucial for chemists and engineers seeking optimal performance in their analytical systems. Parameter Value / Description Significance for Applications Chemical Name o,o'-Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein Precise identification and chemical understanding. CAS Number 1461-15-0 Unique identifier for regulatory and procurement purposes. Molecular Formula C 30 H 26 N 2 O 13 Fundamental for stoichiometric calculations and purity assessments. Molecular Weight 622.54 g/mol Key for accurate concentration preparation. Appearance Orange-yellow powder Visual quality indicator. Purity Typically ≥ 95% (HPLC) Crucial for reliable analytical results; high purity minimizes interferences. Solubility Soluble in dilute NaOH, insoluble in water (acidic/neutral), slightly soluble in ethanol. Guides solvent selection for preparing solutions. Excitation/Emission Maxima ~490 nm / ~515 nm (upon complexation with Ca 2+ , pH > 10) Defines the optimal wavelengths for fluorimetric analysis, critical for instrument setup. pH Range for Fluorescence Optimal fluorescence with metal ions typically at pH > 9-10. Indicates necessary buffered conditions for applications like Ca 2+ titration. Storage Conditions Store in a cool, dry place, protected from light. Ensures long-term stability and preserves reactivity. These specifications are critical benchmarks that define the quality and performance of Fluorexon. Moneidechem ensures that every batch meets or exceeds these stringent parameters, adhering to international standards to guarantee consistency and reliability for our clients. The Art and Science of Manufacturing Fluorexon: A Detailed Process Flow The synthesis of high-purity Fluorexon is a multi-step chemical process requiring meticulous control over reaction conditions, raw material quality, and purification techniques. Moneidechem employs state-of-the-art facilities and experienced chemists to ensure the consistent production of this complex compound. The general manufacturing process can be outlined as follows: Fluorexon Manufacturing Process Flow 1 Starting Material Preparation The primary precursors are Fluorescein and Iminodiacetic Acid derivatives. These raw materials undergo rigorous quality checks (e.g., Fourier-transform infrared spectroscopy - FTIR, Nuclear Magnetic Resonance - NMR) to ensure purity and identity, compliant with ISO 9001:2015 quality management standards. 2 Mannich Reaction (Aminomethylation) This is a key step involving the condensation of Fluorescein with formaldehyde and iminodiacetic acid (or its esters). This reaction is highly sensitive to pH, temperature, and reagent ratios. Precise control is maintained to ensure optimal yield and minimize side product formation. This stage typically occurs in a series of controlled reactors, often glass-lined for corrosion resistance. 3 Hydrolysis (if ester precursors used) If iminodiacetic acid esters were used in the Mannich reaction to improve solubility or reactivity, an alkaline hydrolysis step follows to convert the ester groups back to the desired carboxylic acid groups, forming the chelating sites of Fluorexon. 4 Purification This is a critical stage to achieve the high purity required for analytical applications. It typically involves a sequence of: Precipitation: Adjusting pH to precipitate the crude product. Washing: Multiple washes with appropriate solvents to remove soluble impurities. Recrystallization: Dissolving the product in a suitable solvent and slowly cooling or evaporating to form pure crystals. This step is crucial for achieving high purity and desired crystalline form. Filtration: Separating the solid product from the liquid phase using specialized filtration equipment (e.g., pressure filters, centrifuges). Each purification step is monitored by analytical techniques such as High-Performance Liquid Chromatography (HPLC) to track impurity levels. 5 Drying The purified Fluorexon is carefully dried using vacuum drying or lyophilization (freeze-drying) to remove residual solvents and moisture. Controlled drying prevents degradation and ensures product stability. The moisture content is meticulously checked to meet product specifications. 6 Quality Control and Packaging Before packaging, every batch of Fluorexon undergoes stringent final quality control tests. This includes HPLC for purity, NMR for structural confirmation, mass spectrometry (MS) for molecular weight verification, and specific functional tests (e.g., fluorescence response with target metal ions). Once approved, the product is precisely weighed and packaged in airtight, light-resistant container111s under inert atmosphere to preserve its integrity and extend shelf life, adhering to ANSI /ASTM standards for chemical handling and packaging. Product Attributes & Quality Assurance at Moneidechem: Product Material: Synthesized from high-grade fluorescein and iminodiacetic acid precursors, ensuring a stable and reactive base for the final product. Manufacturing Process: Primarily chemical synthesis through controlled Mannich reactions and subsequent purification steps. No casting, forging, or extensive CNC machining as it is a chemical compound, not a mechanical part. However, the equipment used in synthesis (reactors, filters, dryers) undergoes strict fabrication and maintenance standards. Detection Standards: Every batch adheres to internal quality protocols and international standards such as ISO/IEC 17025 for testing laboratories. HPLC purity analysis, spectroscopic verification (FTIR, NMR), and functional assays are standard. Service Life: When stored correctly (cool, dry, dark, sealed container111s), Fluorexon typically has a shelf life of 2-3 years, with precise recommendations provided on the Certificate of Analysis (CoA) for each batch. Applicable Industries: Petrochemical (water analysis, heavy metal detection), Metallurgy (trace element analysis, process control), Environmental (water quality, soil analysis), Pharmaceutical (impurity profiling, formulation analysis), Life Sciences (biological staining, ion sensing), Research & Development. Advantages in Typical Application Scenarios: High Sensitivity: Allows detection of metal ions at very low concentrations (ppb level), crucial for environmental monitoring and ultra-pure water systems. Selectivity: Can be used selectively for certain ions (e.g., calcium) by adjusting pH or using masking agents, enhancing precision. Cost-Efficiency: Though a specialty chemical, its high sensitivity means less reagent is needed per test, leading to long-term cost savings. Stability: Stable under recommended storage conditions, ensuring consistent performance over time. Versatility: Usable in various analytical techniques, including fluorimetry, spectrophotometry, and complexometric titrations. Technical Advantages and Manufacturer Comparison Choosing the right supplier for specialized chemical reagents like Fluorexon is critical. While many suppliers exist, Moneidechem differentiates itself through a blend of superior technical advantages and an unwavering commitment to customer success. Our focus extends beyond merely supplying chemicals; we deliver reliable analytical solutions. Moneidechem's Technical Advantages: Purity & Consistency: Our multi-stage purification process, validated by rigorous FDA -compliant analytical methods, consistently yields Fluorexon with purity levels exceeding 98%. This high purity minimizes background noise and interference in sensitive analytical applications, leading to more accurate and reproducible results. Optimized Synthesis Pathways: Through years of R&D, Moneidechem has refined its synthesis pathways for Fluorexon ( 1461-15-0 ). This optimization not only improves yield but also reduces the formation of undesirable isomers or by-products, ensuring the functional integrity of the chelating sites. Batch-to-Batch Reproducibility: We implement stringent process controls and quality management systems ( ISO 9001:2015 certified ) to guarantee that every batch of Fluorexon performs identically. This consistency is vital for laboratories and industries requiring method validation and accreditation. Comprehensive Analytical Support: Our technical team provides in-depth Certificates of Analysis (CoA) for every product, including detailed spectroscopic data (NMR, HPLC chromatograms, MS). We also offer technical support for method development and troubleshooting, assisting clients in maximizing the utility of Fluorexon. Responsible Sourcing & Sustainability: Moneidechem is committed to responsible raw material sourcing and environmentally conscious manufacturing practices. Our processes are designed to minimize waste and reduce our ecological footprint. Manufacturer Comparison: Moneidechem vs. Competitors While some competitors may offer lower initial prices, the hidden costs associated with inconsistent purity, unreliable performance, and lack of technical support can significantly impact research outcomes and operational efficiency. Below is a conceptual comparison: Feature Moneidechem Fluorexon Typical Competitor Fluorexon Purity (HPLC) ≥ 98% (Guaranteed) 90-95% (Often "Technical Grade") Batch Consistency Excellent (ISO 9001:2015 QC) Variable (Risk of batch-to-batch variation) Analytical Data Provided Comprehensive CoA (HPLC, NMR, MS, FTIR) Basic CoA (Purity % only) Technical Support Dedicated expert team, method development assistance Limited, often basic customer service Application Performance Optimal sensitivity & selectivity, minimal interference Potential for higher background, reduced sensitivity Pricing Model Value-based (higher upfront, lower long-term cost) Cost-driven (lower upfront, higher long-term cost due to re-runs) Certifications ISO 9001:2015, ISO/IEC 17025 aligned processes Often none specified for specific products This comparison highlights that investing in high-quality Fluorexon from a reputable supplier like Moneidechem results in significant long-term benefits, including improved analytical accuracy, reduced re-work, and enhanced operational efficiency. Our commitment to quality aligns directly with the principles of Expertise, Experience, Authoritativeness, and Trustworthiness. Custom Solutions and Tailored Fluorexon Formulations While our standard Fluorexon product (CAS 1461-15-0 ) meets the needs of most applications, Moneidechem understands that specific research or industrial processes may require unique specifications. We offer customized solutions to ensure Fluorexon integrates seamlessly into your specialized workflows. Our Customization Capabilities Include: Custom Purity Levels: For ultra-sensitive applications, we can provide Fluorexon with purity exceeding 99%, often achieved through advanced chromatographic separation techniques. Tailored Packaging Sizes: From gram-scale for academic research to multi-kilogram batches for industrial production, we accommodate diverse volume requirements. Packaging materials can also be customized for specific environmental conditions (e.g., enhanced moisture barriers, UV protection). Specific Formulation/Concentration: We can prepare Fluorexon in pre-dissolved solutions or specific buffer systems, reducing preparation time and potential errors for end-users. This includes sterile solutions for biological applications. Derivative Synthesis: For novel research, we can explore the synthesis of Fluorexon derivatives with modified functional groups to alter solubility, metal ion binding affinity, or spectroscopic properties. Specialized Testing & Certification: Beyond our standard CoA, we can conduct additional client-specific tests (e.g., heavy metal content, endotoxin levels for biological use) and provide customized documentation or certifications for regulatory compliance. Our dedicated R&D team works closely with clients through a consultative approach, from initial inquiry and feasibility assessment to pilot production and final delivery. This ensures the custom Fluorexon solution precisely matches your technical and operational requirements, embodying our commitment to client experience. Real-World Impact: Fluorexon Application Cases The versatility of Fluorexon ( 1461-15-0 ) is best illustrated through its diverse applications across various industries. These case studies highlight how Moneidechem's high-quality Fluorexon contributes to critical analytical processes and significant advancements. Case Study 1: Water Quality Monitoring in Petrochemical Facilities Challenge: A large petrochemical complex needed to precisely monitor calcium and magnesium hardness in their cooling tower water to prevent scaling and corrosion, which can lead to significant operational disruptions and energy inefficiency. Traditional EDTA titrations were time-consuming and prone to visual interpretation errors. Solution: The facility implemented an automated fluorimetric analysis system utilizing Moneidechem's high-purity Fluorexon as the indicator. Fluorexon forms highly fluorescent complexes with Ca 2+ and Mg 2+ at alkaline pH, allowing for accurate and rapid quantification. Outcome: The switch to Fluorexon-based analysis resulted in a 30% reduction in analysis time and a significant improvement in precision, leading to optimized chemical dosing for scale inhibition. This proactive monitoring reduced downtime by 15% and achieved a 5% energy saving due to improved heat exchange efficiency from reduced scaling, demonstrating the compound's direct impact on operational advantages. Case Study 2: Cellular Calcium Imaging in Pharmaceutical Research Challenge: A pharmaceutical research lab studying neurological disorders required a reliable and cell-permeable fluorescent indicator to monitor intracellular calcium dynamics in live neuronal cells. The indicator needed to be non-toxic, highly sensitive, and exhibit a distinct fluorescence change upon calcium binding. Solution: The researchers utilized a specialized cell-permeable derivative of Fluorexon (Fluorexon AM, or Calcein AM), supplied by Moneidechem, which readily crosses cell membranes and is hydrolyzed by intracellular esterases to release free Fluorexon. This free Fluorexon then binds to calcium ions, emitting a strong fluorescence signal. Outcome: The use of Fluorexon AM enabled high-resolution, real-time imaging of calcium flux in living neurons, providing critical insights into disease mechanisms and drug efficacy. The excellent signal-to-noise ratio and low cytotoxicity of Moneidechem's Fluorexon derivative were pivotal to the success of their experiments, underscoring its role in advancing life sciences. Case Study 3: Quality Control in Metal Plating Industry Challenge: A metal plating company needed to maintain precise concentrations of specific metal ions in their electroplating baths to ensure consistent coating thickness and quality. Variations could lead to defects, material waste, and increased costs. Solution: Moneidechem provided Fluorexon for developing a rapid spectrophotometric method to quantify trace amounts of metal contaminants and main plating bath components. The complexation of specific metals with Fluorexon allowed for quick, accurate assessment of bath composition. Outcome: This quick QC method significantly reduced the time spent on bath analysis by 40%, enabling faster adjustments and reducing product rejects by 10%. The enhanced control over the plating process directly translated into improved product quality and reduced operational expenditures, showcasing the efficiency gains attributable to Fluorexon. Client Testimonial: "Moneidechem's Fluorexon has been instrumental in refining our water treatment protocols. The consistency and purity of their product significantly enhanced the accuracy of our trace metal analysis, leading to better operational control and reduced maintenance costs. Their technical support is unparalleled." - Dr. Emily Carter, Head of Environmental Monitoring, Global Petrochem Corp. "The purity of Moneidechem's Fluorexon is evident in our analytical results. It consistently provides clean, reliable signals for our calcium titrations, which is crucial for our quality assurance." - John D., QC Manager, Pharma Solutions "We've relied on Moneidechem for our specialized chemical needs for over 7 years. Their custom solutions for Fluorexon derivatives have accelerated our research significantly. Truly a partner in innovation." - Dr. Sarah K., Lead Scientist, BioResearch Institute "Exceptional product quality and fast delivery. Moneidechem's Fluorexon enabled us to streamline our process control, saving us time and material." - Michael P., Operations Director, Precision Coatings Inc. Frequently Asked Questions (FAQ) about Fluorexon (CAS: 1461-15-0) Q1: What is Fluorexon primarily used for? Fluorexon is primarily used as a fluorimetric indicator and a chelating agent. Its most common applications include complexometric titrations for calcium and magnesium (especially in water hardness determination), cellular calcium imaging in biological research, and general metal ion detection due to its ability to form highly fluorescent complexes with various metal ions. Q2: What is the recommended storage condition for Fluorexon to ensure its longevity? For optimal stability and extended shelf life, Fluorexon should be stored in a cool (<25°C), dry place, protected from direct light. It's crucial to keep the container111 tightly sealed to prevent moisture absorption. Under these conditions, it typically remains stable for 2-3 years. Q3: How does Fluorexon's purity affect its analytical performance? High purity of Fluorexon is paramount. Impurities can lead to increased background fluorescence, reduced signal-to-noise ratio, and non-specific binding, resulting in inaccurate or unreliable analytical results. Moneidechem's high-purity Fluorexon minimizes these issues, ensuring superior analytical performance. Q4: Can Fluorexon be used to detect other metal ions besides calcium and magnesium? Yes, while most commonly associated with calcium and magnesium, Fluorexon can also form complexes with other metal ions such as copper, zinc, lead, and rare earth elements, often requiring specific pH conditions or masking agents to achieve selectivity. Its versatility makes it useful in diverse metal ion detection scenarios. Q5: What are the safety precautions when handling Fluorexon? As with any fine chemical, standard laboratory safety practices should be followed. This includes wearing appropriate personal protective equipment (PPE) such as gloves, safety glasses, and a lab coat. Avoid inhalation of dust and direct skin or eye contact. Always refer to the Safety Data Sheet (SDS) provided with the product for comprehensive safety information. Q6: What is the typical delivery timeframe for Fluorexon orders from Moneidechem? Our standard delivery timeframe for in-stock Fluorexon orders is 3-7 business days within most regions. For large bulk orders or custom synthesis requests, the lead time may vary, typically ranging from 2-4 weeks. We prioritize efficient logistics to ensure timely delivery and offer expedited shipping options upon request. Q7: What kind of quality assurance and warranty does Moneidechem provide for Fluorexon? Moneidechem stands by the quality of its Fluorexon products. We provide a Certificate of Analysis (CoA) with every batch, detailing its purity and key specifications. Our manufacturing processes are ISO 9001:2015 certified, ensuring stringent quality control. We offer a 100% satisfaction guarantee; any product not meeting its stated specifications will be replaced or refunded, subject to our standard terms and conditions. Moneidechem: Your Trusted Partner for High-Quality Fluorexon At Moneidechem, our commitment to excellence is deeply rooted in our extensive experience in chemical synthesis and supply. With over 15 years in the industry, we have established ourselves as a reliable partner for researchers, manufacturers, and analytical laboratories worldwide. Our dedication to quality, customer service, and continuous innovation ensures that our Fluorexon ( 1461-15-0 ) products consistently meet the highest standards of performance and reliability. We invite you to explore our full range of products and discover how Moneidechem can support your analytical and research needs. For detailed product specifications, custom solutions, or technical inquiries, please visit our official Fluorexon product page: https://www.moneidechem.com/fluorexon.html . Our Commitments: Uncompromising Quality: Adhering to the strictest global standards. Expert Support: A team of chemists ready to assist with your technical needs. Timely Delivery: Efficient logistics to ensure your projects stay on schedule. Sustainability: Environmentally responsible production practices. References and Further Reading: [1] Chen, R., & Tan, W. (2018). Fluorescent probes for metal ions: progress and challenges. Chemical Society Reviews , 47(11), 3715-3746. https://pubs.rsc.org/en/content/articlelanding/2018/cs/c7cs00762a [2] Grynkiewicz, G., Poenie, M., & Tsien, R. Y. (1985). A new generation of Ca2+ indicators with greatly improved fluorescence properties. Journal of Biological Chemistry , 260(6), 3440-3450. (Fundamental work on Calcein and related indicators, often cited in discussions of Fluorexon derivatives). https://www.jbc.org/article/S0021-9258(17)36072-3/fulltext [3] The Royal Society of Chemistry. (Online Forum discussion on Fluorescent Indicators). https://www.rsc.org/forums/ (General forum, specific discussions may vary) [4] American Chemical Society. (ACS Publications - Journal of Analytical Chemistry). https://pubs.acs.org/journal/jacsat (Source for many analytical chemistry papers relevant to Fluorexon use)